Nyren’s sequencing method earned the name “pyrosequencing” since it revolved around the production of pyrophosphate. At first, pyrosequencing could sequence only short DNA snippets, with a few nucleotides. In 1996, however, Nyren’s lab demonstrated sequencing of up to 15 bases by using a modified “A” nucleotide to reduce their signal-to-noise ratio.7 In 1998, they increased this to 34 bases by adding another enzyme, called apyrase, to the mix; apyrase degraded unincorporated nucleotides, removing the need for constant wash steps.
Agar comes from multiple sources, as many red seaweeds are “agarophytes” (that is, seaweeds containing agar in their cell walls). Species of Gelidium are the most important source of bacteriological (lab-grade) agar. Other main agarophytes, largely used for culinary agar, include red seaweeds from the genera Gracilaria, Pterocladia, Ahnfeltia, and others. Species from the genera Eucheuma, Gigartina, and Chondrus have been used as agarophytes in research during agar shortages.9，这一点在同城约会中也有详细论述
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Linda Cho, The Great Gatsby，详情可参考WPS下载最新地址